In-vitro Antibacterial and Antibiofilm Activity of Cinnamomum verum Leaf Oil against Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae

Phytomedicines are becoming more popular in treatment of infectious diseases worldwide. Cinnamomum verum essential oil (EO) has been used as a therapeutic alternative for various diseases. This study aimed to evaluate the antibacterial and antibiofilm activity C. leaf EO against Pseudomonas aeruginosa, Staphylococcus aureus Klebsiella pneumoniae. Effect vapor on planktonic cells was determined using microatmosphere technique. CLSI M7-A10 method employed Minimum Inhibitory Concentration (MIC) Bactericidal (MBC) experiments. established biofilms quantified visualized XTT Scanning Electron Microscope (SEM). In-vitro toxicity evaluated Human Keratinocytes (HaCaT). Chemical analysis done Gas Chromatography- Mass Spectrometry (GC-MS). All tested strains were sensitive cinnamon vapor. exhibited 0.5 1.0 mg/mL MIC MBC all test strains. Biofilm Eradication Concentrations (MBIC50 MBEC) 4.0 mg/mL. SEM indicated cellular shrinkages, cell wall damages, decreased biofilm densities. Cinnamon didn't show any HaCaT at concentration tested. Eugenol most abundant compound oil. shows an with minimal host.